Electrochemical Biosensor Utilizing Supramolecular Association of Enzyme on Sol−gel Matrix Embedded Gold Nanoparticles Supported Reduced Graphene Oxide−cyclodextrin Nanocomposite |
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Authors: | Shanmugam Manivannan Kyuwon Kim |
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Affiliation: | Electrochemistry Laboratory for Sensors & Energy (ELSE), Department of Chemistry, Incheon National University, Republic of Korea |
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Abstract: | Herein we present β‐cyclodextrin (CD)‐functionalized reduced graphene oxide (RGO) nanosheets supported on silicate sol‐gel matrix‐embedded gold nanoparticles (Au NPs) modified electrode as a new affinity binding nanocomposite. The modified electrode is fabricated through layer‐by‐layer drop casting followed by immobilization of chemically modified enzyme conjugate (horse radish peroxidase (HRP)?adamantane carboxylic acid (ADA)). This affinity system is based on the supramolecular association between CDs and HRP?ADA and is mimicking the biological avidin?biotin interactions. CDs‐functionalized RGO (RGO?CD) functions as a macrocyclic host to form stable supramolecular inclusion complexes with enzyme conjugate. Besides Au NPs improve the interfacial interaction with RGO?CD nanosheets, and thus exhibit synergistic electrocatalytic effect toward H2O2 reduction in the presence of 1 mM hydroquinone. |
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Keywords: | Electrochemical biosensor Cyclodextrin Enzyme immobilization Graphene Gold Supramolecular assembly |
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