Circular dichroism study of enzymatic manipulation on magnetic and metallic DNA template nanowires |
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| Affiliation: | 1. Weldon School of Biomedical Engineering, Purdue University, West Lafayette, cityIN 47906, USA;2. Department of Chemistry, Purdue University, West Lafayette, cityIN 47906, USA;1. Department of Medical System Engineering, Gwangju Institute of Science and Technology, 1 Oryong-dong Buk-gu, Gwangju 500-712, Republic of Korea;2. Department of Surgery, Chonnam National University Medical School, 8 Hak-dong, Dong-gu, Gwangju 501-757, Republic of Korea;3. Department of Dermatology, Chonnam National University Medical School, 8 Hak-dong, Dong-gu, Gwangju 501-757, Republic of Korea;4. School of Life Sciences, Gwangju Institute of Science and Technology, 1 Oryong-dong Buk-gu, Gwangju 500-712, Republic of Korea;5. Advanced Photonics Research Institute, Gwangju Institute of Science and Technology, 1 Oryong-dong Buk-gu, Gwangju 500-712, Republic of Korea;1. J. Haber Institute of Catalysis and Surface Chemistry Polish Academy of Sciences, Niezapominajek 8, 30-239 Krakow, Poland;2. Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, Łojasiewicza 11, 30-348 Krakow, Poland;1. State Key Laboratory of Metastable Materials Science and Technology, Yanshan University, Qinhuangdao 066004, China;2. Department of Materials Science and Engineering, College of Engineering, Peking University, Beijing 100871, China;3. Department of Materials Physics and Chemistry, University of Science and Technology Beijing, Beijing 100083, China;1. Graduate Schools of Pharmaceutical Sciences, Nagoya, Japan;2. Medical Sciences, Nagoya City University, Nagoya, Japan;3. Nutritional Health Science Research Center, Chubu University, Kasugai, Japan;1. School of Physics, Shandong University, Jinan 250100, China;2. School of Science, Shandong Jianzhu University, Jinan 250101, China |
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| Abstract: | Circular dichroism spectroscopy (CD) was used to examine the mechanism of endonuclease clipping and ligation of the DNA template nanowires. The biomolecular manipulation of the DNA template is compared for both metallic (Au) and magnetic (Fe2O3 and CoFe2O4) nanowires. The dependence of nanoparticle (NP) concentration on enzymatic clipping and DNA ligation was studied, in addition to performing absorbance and thermal melting experiments. Low-NP concentration preserved and digested the DNA template structure. Yet, at higher NP concentrations, the DNA template began to denature before enzyme addition. It was also observed that ligation of the digested DNA occurred more efficiently at low-NP concentrations. These results provide significant information on structural alteration and biorecognition effectiveness of the DNA template after enzymatic manipulation. |
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