Methotrexate-incorporated polymeric nanoparticles of methoxy poly(ethylene glycol)-grafted chitosan |
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| Institution: | 1. Department of Physics, Manonmaniam Sundaranar University, Tirunelveli 627 012, India;2. PG & Research Department of Physics, Sri Paramakalyani College, Alwarkurichi 627 412, India;3. Materials Science Division, Indira Gandhi Centre for Atomic Research, Kalpakkam, India;1. Department of Bone and Soft Tissue Tumor Surgery, Cancer Hospital of China Medical University, Liaoning Cancer Hospital & Institute, Shenyang, Liaoning Province 110042, China;2. The Graduate School, Dalian Medical University, Dalian, Liaoning Province 116044, China;1. Rapid Prototype & Electrospinning Lab, Department of Metallurgical and Materials Engineering, DIAT (DU), Ministry of Defence, Girinagar, Pune 411025, Maharashtra, India;2. NanoEngineering Lab, Department of Metallurgical and Materials Engineering, Indian Institute of Technology Kharagpur, 721302 West Bengal, India;3. Nanomaterials Characterization Lab, Center for Converging Technologies, University of Rajasthan, JLN Marg, Jaipur 302017, Rajasthan, India;1. CQFM—Centro de Química-Física Molecular and IN—Institute of Nanoscience and Nanotechnology, Instituto Superior Técnico, Universidade de Lisboa, 1049-001 Lisboa, Portugal;2. CQE—Centro de Química Estrutural, Complexo I, IST, Universidade de Lisboa, 1049-001 Lisboa, Portugal;1. Department of Chemistry, Materials and Chemical Engineering “Giulio Natta”, Politecnico di Milano, Milano, Italy;2. Department of Chemistry and Applied Biosciences, Institute for Chemical and Bioengineering, ETH Zurich, Zurich, Switzerland |
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| Abstract: | We prepared methotrexate (MTX)-encapsulated polymeric nanoparticles using methoxy poly(ethylene glycol) (MPEG)-grafted chitosan (ChitoPEG) copolymer. MTX-encapsulated polymeric nanoparticles of ChitoPEG copolymer has around 50–300 nm in particle size and showed spherical shape when observed by transmission electron microscope (TEM). In 1H nuclear magnetic resonance (NMR) study, the specific peaks of MTX and chitosan as a drug carrying inner-core disappeared at D2O and only the specific peak of MPEG was observed, while specific peaks of MPEG, MTX, and chitosan appeared in DCl/D2O mixtures. These results indicated that MTX was complexed with chitosan and then core–shell type nanoparticles had formed in aqueous environment, i.e., MTX/chitosan complexes composed of inner-core and MPEG composed of outer-shell of the nanoparticles. Loading efficiency of MTX in the polymeric nanoparticles was 94% (w/w) of ChitoPEG-1, 91.1% (w/w) of ChitoPEG-2, 90.1% (w/w) of ChitoPEG-3 and 65.2% (w/w) of ChitoPEG-4, expectively. The higher the drug feeding ratio, the higher the drug content and the lower the loading efficiency. The higher the MPEG graft ratio in the copolymer, the lower the drug content and loading efficiency. Drug contents evaluated by 1H NMR were the same as found by UV spectrophotometer. |
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