The elastic microstructure of various tissues

Previous work has indicated that a modified Quate-Lemons scanning acoustic microscope (SAM) is capable of measuring the acoustic propagation properties of sections of biological tissue. The lens is excited by an impulse, rather than a tone burst, and the undemodulated returning signal from the tissue is recorded. The variations in received signal with time are used to deduce the sound speed, attenuation, impedance, and section thickness. In this article, the technique is applied to various types of tissue, and the variations in acoustic propagation properties are computed. Conventional tone burst SAM images at 425 MHz are compared with the time resolved data in order to elucidate the contrast mechanisms. The effects of varying the frequency and position of the focal plane on the tone burst images are interpreted in the light of the broadband results.