基于表面等离子体子共振的B因子传感器

以分辨率较高的一米光栅单色仪为分光系统,光电倍增管为检测系统,改进了自行组装的表面等离子体子共振(SPR)传感装置,提高了仪器的检测能力约50倍,对于发展改变波长模式的SPR传感器具有重要意义。以对金和抗体均有较强吸附作用的葡萄球菌A蛋白为基底膜,观测了人的B因子抗体和抗原之间免疫反应的动力学过程,并研究了B因子的定量测定。结果表明,B因子抗原的浓度在0.02～5μg/mL范围内与信号的响应值呈线性关系。该传感器灵敏度高,选择性和重现性均好。

An Immunosensor of B Factor Based on Surface Plasmon Resonance

Anovel optical immunosensor based on surface plasmon resonance(SPR) has been developed for identification of antigen. The sensor is designed on the basis of fixing angle of incidence and measuring the reflected intensities in the wavelength range of 200-800 nm. Molecular self-assembling in solution is used to form the sensing membrane on gold substrate. The kinetic processes of sensing monolayer formation were studied. Human Bfactor (Bf), an activator of complement C3, was detected in the concentration range from 0.02-5 μg/mL. Under the selected experimental conditions, the sensor has a good sensitivity, repeatability, reversibility and selectivity.