PHYTOCHROME CONTROL OF EXTRACELLULAR PEROXIDASE ACTIVITY IN MUSTARD INTERNODES: CORRELATION WITH GROWTH, and COMPARISON WITH THE EFFECT OF WOUNDING

Abstract— The effects of phytochrome status on extracellular peroxidase activity were investigated in Sinapis alba L. seedlings grown for 12 days under continuous white light and transferred to darkness after a red light or a far-red light pulse. The rates of extension growth and dry matter accumulation in the first internode were increased by the far-red light pulse. Extracellular proteins, obtained by low speed centrifugation of intact internodes infiltrated with CaCl₂, were separated by isoelectrofocusing, and four extracellular acidic peroxidases were resolved, the most active being A3 and A4 (both ˜60 kD). The activity of A4 was reduced by the far-red light pulse perceived by phytochrome, while the activity of A3 was unaffected. The promotion of internode extension growth caused by far-red light is biphasic [Casal and Smith (1989) Plant, Cell Environ. 12 ,511–50]. Changes in peroxidase activity were detected prior to the second, but not to the first phase of the internode growth promotion. The effects on both growth and peroxidase activity were virtually restricted to the upper half of the internode and, once established, did not subsequently increase in magnitude.
In contrast to the effects mediated by phytochrome, blue light pretreatments affected growth but not extracellular peroxidase activity. Wounding the internode reduced extension growth, increased the activity of A3, but caused no significant effects on A4.
Other extracellular proteins, separated in sodium dodecyl sulphate polyacrylamide gels and stained with Coomassie blue, showed no significant differences. The concentration of extracellular proteins was higher in the upper than the lower half of the internode.
Results are discussed in terms of phytochrome effects in light grown plants, peroxidase activitv-arowth relationships, and extracellular peroxidase isoform functions.