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Spectrofluorimetric method for the determination of uric acid in human serum |
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| Authors: | W. W. Bian L. Wang N. Zhang C. Q. Jiang |
| Affiliation: | 1. Department of Medical Chemistry, Weifang Medical University Weifang, 261053, Weifang, P.R. China 2. State Key Laboratory of Ceramic, Zibo Entry and Exit Inspection and Quarantine Bureau Zibo, 255000, Zibo, P.R. China 3. Department of Chemistry, Huangshan University, Huangshan, 245041, P.R. China 4. Department of Chemistry, Shandong Normal University, Jinan, 250014, P.R. China |
| Abstract: | A new spectrofluorimetric method is described for the determination of uric acid (UA), that can remarkably reduce the fluorescence intensity of the enoxacin (ENX)-terbium ion (Tb3+) complex at 545 nm. The reduced fluorescence intensity of Tb3+ ion at pH 5.7 is proportional to the concentration of UA. Optimum conditions for the determination of UA have been investigated. The linear range and detection limit for the determination of UA are 6.0 × 10?7–3.0 × 10?5 M and 1 × 10?7 M, respectively. The relative standard deviation (RSD) was 0.4% for 6 × 10?6 M UA (n = 11). The method is simple, practical and relatively free of interferences. It has been successfully applied to assess UA in serum at the level of 3 × 10?4 M with an RSD of 5–7% (n = 3). The results were evaluated by comparison with a common clinical spectrophotometric method using phosphotungstic acid as developer. |
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