| TRANSFER OF THE ATRAZINE-RESISTANT GENE OF BLACK NIGHTSHADE TO SOYBEAN CHLOROPLAST GENOME AND ITS EXPRESSION IN TRANSGENIC PLANTS |
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| 引用本文： | 刘博林,岳绍先,胡乃壁,李小兵,翟文学,李诺,朱荣焕,朱立煌,毛大璋,周佩珍. TRANSFER OF THE ATRAZINE-RESISTANT GENE OF BLACK NIGHTSHADE TO SOYBEAN CHLOROPLAST GENOME AND ITS EXPRESSION IN TRANSGENIC PLANTS[J]. 中国科学B辑(英文版), 1990, 0(4) |
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| 作者姓名： | 刘博林 岳绍先 胡乃壁 李小兵 翟文学 李诺 朱荣焕 朱立煌 毛大璋 周佩珍 |
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| 作者单位： | nstisute of Crop Breeding and Cultivation，Chinese Academy of Agricultural Sciences，Beijing 100081，PRC，nstisute of Crop Breeding and Cultivation，Chinese Academy of Agricultural Sciences，Beijing 100081，PRC，Institute of Genetics，Academia Sinica，Beijing 100012，PRC，Institute of Genetics，Academia Sinica，Beijing 100012，PRC，Institute of Genetics，Academia Sinica，Beijing 100012，PRC，Institute of Genetics，Academia Sinica，Beijing 100012，PRC，Institute of Genetics，Academia Sinica，Beijing 100012，PRC，Institute of Botany，Academia Sinica，Beijing 100044，PRC，Institute of Botany，Academia Sinica，Beijing 100044，PRC，Institute of Botany，Academia Sinica，Beijing 100044，PRC |
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| 摘 要： | The atrazine-resistant psbA gene of black nightshade was transferred to the chloroplast genome of atrazine-susceptible soybean by means of ovary microinjection during the stage of zygote. The identification was carried out by using the methods of spraying the leaves directly with atrazine solution, examining the change of leaf fluorescence kinetics under a brighter light induction, molecular hybridization, etc. The experimental results show that the transgenic soybean plants do have been obtained for the first time.
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TRANSFER OF THE ATRAZINE-RESISTANT GENE OF BLACK NIGHTSHADE TO SOYBEAN CHLOROPLAST GENOME AND ITS EXPRESSION IN TRANSGENIC PLANTS |
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| LIU BO-LIN,,YUE SHAO-XIAN. TRANSFER OF THE ATRAZINE-RESISTANT GENE OF BLACK NIGHTSHADE TO SOYBEAN CHLOROPLAST GENOME AND ITS EXPRESSION IN TRANSGENIC PLANTS[J]. Science in China(Chemistry), 1990, 0(4) |
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| Authors: | LIU BO-LIN YUE SHAO-XIAN |
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| Affiliation: | LIU BO-LIN,*,YUE SHAO-XIAN, |
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| Abstract: | The atrazine-resistant psbA gene of black nightshade was transferred to the chloroplast genome of atrazine-susceptible soybean by means of ovary microinjection during the stage of zygote. The identification was carried out by using the methods of spraying the leaves directly with atrazine solution, examining the change of leaf fluorescence kinetics under a brighter light induction, molecular hybridization, etc. The experimental results show that the transgenic soybean plants do have been obtained for the first time. |
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| Keywords: | atrazine psbA gene transgenic soybean plant. |
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