A STUDY OF THE FLASH INDUCED 518 NM ABSORBANCE CHANGE KINETICS UNDER ANAEROBIC CONDITIONS |
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| Authors: | Radovan Popovic Doug Bruce Konrad Colbow William Vidaver |
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| Institution: | Photobiology Group, Simon Fraser University, Burnaby, B.C. Canada V5A 1S6 |
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| Abstract: | The preillumination induced acceleration of the flash-induced 518 nm absorbance change (ΔA518) decay was studied in lettuce leaves and chloroplasts. In leaves, the acceleration was inhibited by DCMU or reversibly by removal of oxygen. In chloroplasts with added ADP and phosphate and/or reconstructed electron transport, the acceleration was also inhibited by DCMU or the lack of O2.
Anaerobic inhibition of ΔA518 decay acceleration was no longer observed when hydroxylamine replaced water as electron donor to PSII. Anaerobiosis was also shown to reversibly inhibit the initial rate of FeCN reduction in chloroplasts. These results suggest the mechanism of anaerobic inhibition of ΔA518 decay acceleration to be associated with the O2 evolving system. |
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