Extraction and characterization of native heteroxylans from delignified corn stover and aspen |
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Authors: | Radnaa Naran Stuart Black Stephen R Decker Parastoo Azadi |
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Institution: | (1) Complex Carbohydrate Research Center, 315 Riverbend Road, Athens, GA 30602-4712, USA;(2) Chemical and Biosciences Center, National Renewable Energy Laboratory (NREL), 1617 Cole Blvd MS 3323, Golden, CO 80401, USA |
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Abstract: | Dimethylsulfoxide-solubilized polysaccharides from delignified corn stover and aspen were characterized. The biomass was delignified
by two different techniques; a standard acid chlorite and a pulp and paper QPD technique comprising chelation (Q), peroxide
(P), and acid-chlorite (D). Major polysaccharides in all fractions were diversely substituted xylan. Xylan acetylation was
intact after chlorite delignification and, as expected, xylan from QPD-delignified fraction was de-acetylated by the alkaline
peroxide step. The study of DMSO-extractable xylans from chlorite-delignified biomass revealed major differences in native
acetylation patterns between corn stover and aspen xylan. Xylan from cell walls of corn stover contains 2-O- and 3-O-mono-acetylated xylan and MeGlcA-α-(1 → 2)]3-OAc]-xylp units. In addition, aspen xylan also contains 2,3-di-O-acetylated xylose. 1,4-β-d-xylp residues substituted with MeGlcA at O-2 position are absent in chlorite-delignified aspen xylan. Sugar composition in accord with NMR-spectroscopic data indicated
that corn stover xylan is arabinosylated while aspen xylan is not. We have shown that corn stover xylan has similar structure
with xylans from other plants of Poales order. No evidence was found to indicate the presence of 1,4-β-d-MeGlcA-α-(1 → 2)]Ara-α-(1 → 3)]-xylp in corn stover xylan fractions. |
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Keywords: | Biomass Biomass delignification Plant cell wall Glucuronoxylan Hardwood xylan Corn stover xylan Xylan acetylation Endoxylanase Homo- and-heteronuclear NMR-spectroscopy MALDI-TOF MS |
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