Polyamide resin: A novel immunoadsorbent |
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Authors: | Gary T Gooch Sumner I Schwarz Jonathan L Kiel Johannes Everse |
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Institution: | 1. Department of Microbiology, Texas Tech University Health Sciences Center, School of Medicine, 79430, Texas, Lubbock, USA 2. Biochemistry, Texas Tech University Health Sciences Center, School of Medicine, Lubbock, 79430, Texas, USA
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Abstract: | A solid phase assay for radiolabeled antibody synthesizedde novo in vitro has been described (1). The solid phase consists of antigen covalently bound to bromacetyl cellulose, a useful but difficult to prepare immunoadsorbent. Herein, we describe the preparation of polyamide resin immunoadsorbent and the procedure for coupling antigen to the polymer. Data are presented that show that polyamide resin-Ag? conjugates can replace bromacetyl cellulose-Ag conjugates. The usefulness of this easily prepared and inexpensive immunoadsorbent is discussed. |
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