Two new techniques for sample preparation in bioanalysis: Microextraction in packed sorbent (MEPS) and use of a bonded monolith as sorbent for sample preparation in polypropylene tips for 96-well plates |
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Authors: | Lars G. Blomberg |
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Affiliation: | (1) Department of Chemistry and Biomedical Sciences, Karlstad University, SE-651 88 Karlstad, Sweden |
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Abstract: | Analytical methods providing high throughput are required for the ever increasing number of samples in bioanalysis. Currently, the method of choice in bioanalysis is LC-MS-MS. This method is quite rapid and thereby the focus has been directed to sample preparation as being a bottleneck in total analysis systems. It has become necessary to develop sample preparation techniques to a new improved level. This development has been based on a systematic and scientific approach. The key factors in this development have been miniaturization, integration, and automation of the techniques. This review provides a short overview of recent developments. Special emphasis is on two techniques: microextraction in packed syringe (MEPS) and use of a monolithic acrylamide plug as sorbent in polypropylene tips primarily intended for use with 96-well plate systems. |
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Keywords: | Sample preparation Micro-extraction Monolithic sorbent High throughput Automation Mass spectrometry Quantitative analysis Bioanalysis |
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