| 兔金属硫蛋白的分离与纯化 |
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| 引用本文： | 张保林,陶祖贻.兔金属硫蛋白的分离与纯化[J].离子交换与吸附,1994,10(4):289-293. |
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| 作者姓名： | 张保林 陶祖贻 |
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| 作者单位： | 南京大学配位化学研究所，配位化学国家重点实验室，兰州大学现代物理系 |
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| 摘 要： | 兔皮下注射ＣｄＣｌ２诱导金属硫蛋白（Ｍｅｔａｌｌｏｔｈｉｏｎｅｉｎ，简称ＭＴ），取肝脏匀浆，用乙醇─氯仿混合液萃取后经ＳｅｐｈｄｅｘＧ－５０柱分离，得到混合型ＭＴ．进一步用纤维素弱碱性阴离子交换树脂进行拆分，并在ＳｅｐｈａｄｅｘＧ－２５柱上脱盐，得到ＭＴ－１和ＭＴ－２两种亚型．经氨基酸组咸、凝胶电泳及ＨＰＬＣ柱层析等分析表明，所得的两种亚型具有高度的均一性．
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| 关 键 词： | 金属硫蛋白，诱导，分离纯化 |
Isolation and Purification of Rabbit Liver Metallothionein |
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| Zhang Baolin,Huang Hui,Tang Wenxia.Isolation and Purification of Rabbit Liver Metallothionein[J].Ion Exchange and Adsorption,1994,10(4):289-293. |
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| Authors: | Zhang Baolin Huang Hui Tang Wenxia |
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| Abstract: | A simplified procedure for purifing rabbit liver metallothioncin (MT) using filtration and anion exchange chromatography is presented. In the preliminary experiments, rabbits were injected with CdCl2 to induce the synthesis of MT in animal tissues. The obtained livers were homogenized and precipitated with alcohol and heating followed by isolation on Sephadex G-50 and DEAE-Cellulose DE-32 column. The resultant MT-1and MT-2 are shown to be highly homogeneous by analysis of amino acid composition and HPLC. |
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| Keywords: | Induction Metallothionein Isolation and Purification |
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