Comparison of reactivity of alkane hydroxylation with intact cells and cell- free extracts ofMethylosinus trichosporium OB3b |
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| Authors: | Daisuke Sugimori Ryota Ando Ichiro Okura |
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| Institution: | (1) Department of Bioengineering, Tokyo Institute of Technology, Nagatsuta, 226 Yokohama, Japan |
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| Abstract: | Kinetic studies for hydroxylation of a series of alkanes (methane, ethane and propane) with intact cells and cell-free extracts
ofMethylosinus trichosporium OB3b were carried out.K
m values for alkane hydroxylation with cell-free extracts were lower than those with intact cells, suggesting that cytoplasm
plays an important role in the solubility of alkanes to increase their concentration. |
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| Keywords: | Alkane hydroxylation methane monooxygenase Methylosinus trichosporium OB3b |
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