| 胎儿左右大脑差异表达基因片段的克隆 |
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| 引用本文： | 朱天慧,张立冬,许胜利,张杰,刘志霈.胎儿左右大脑差异表达基因片段的克隆[J].南开大学学报,1999,32(3):201-204. |
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| 作者姓名： | 朱天慧 张立冬 许胜利 张杰 刘志霈 |
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| 作者单位： | 南开大学医学院!天津，300071，南开大学医学院!天津，300071，南开大学医学院!天津，300071，南开大学医学院!天津，300071，南开大学医学院!天津，300071 |
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| 摘 要： | 应用抑制性消减杂交（ＳＳＨ）技术从一２４ 周流产男性胎儿的左右大脑ｍ ＲＮＡ 中克隆到了４２ 个左脑特异表达基因的ｃＤＮＡ 片段，并随机挑选１５ 个克隆的测序结果与美国ＮＩＨ 基因库ＥＳＴ进行了同源性分析，同源性范围是６４％ ～１００％ ，其中有５ 个克隆的基因库同源序列与脑有关，其余１０ 个克隆同源于脑以外的其它组织．
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| 关 键 词： | 抑制性消减杂交(SSH) 同源序列 大脑 |
THE CLONING OF DIFFERENTIALLY EXPRESSED GENE FRAGMENTS BETWEEN LEFT BRAIN AND RIGHT BRAIN IN FETUS |
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| Zhu Tianhui,Zhang Lidong,Xu Shengli,Zhang Jie,Liu Zhipei.THE CLONING OF DIFFERENTIALLY EXPRESSED GENE FRAGMENTS BETWEEN LEFT BRAIN AND RIGHT BRAIN IN FETUS[J].Acta Scientiarum Naturalium University Nankaiensis,1999,32(3):201-204. |
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| Authors: | Zhu Tianhui Zhang Lidong Xu Shengli Zhang Jie Liu Zhipei |
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| Abstract: | cDNA clones specific to left brain were isolated from mRNA of a fetal left and right brain using suppression subtractive hybridization (SSH) technique. Homology search was made between the DNA sequences of randomly selected 15 clones and the EST of NIH genebank. There are 64%~100% homologies between them. 5 out of the 15 clones have homologus sequences related to brain, while the rest of 10 clones do not. |
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| Keywords: | suppression subtractive hybridization (SSH) homologous sequences brain |
| 本文献已被 CNKI 维普 等数据库收录！ |
