Metabolic analysis of the cinnamate/monolignol pathway in Carthamus tinctorius seeds by a stable-isotope-dilution method |
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Authors: | Sakakibara Norikazu Nakatsubo Tomoyuki Suzuki Shiro Shibata Daisuke Shimada Mikio Umezawa Toshiaki |
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Institution: | Research Institute for Sustainable Humanosphare, Kyoto University, Uji, Kyoto 611-0011, Japan. |
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Abstract: | The present study established a system for comprehensive metabolic analysis of the cinnamate/monolignol and lignan pathways by the use of a stable-isotope-dilution method. The system was successfully applied to characterization of the pathways in Carthamus tinctorius cv. Round-leaved White maturing seeds in combination with administration of stable-isotope-labelled precursors. Experimental results obtained using this technique strongly suggested the intermediacy of ferulic acid in lignan biosynthesis in the plant. |
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