Single-molecule surface enhanced resonance Raman spectroscopy of the enhanced green fluorescent protein |
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Authors: | Habuchi Satoshi Cotlet Mircea Gronheid Roel Dirix Gunter Michiels Jan Vanderleyden Jos De Schryver Frans C Hofkens Johan |
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Affiliation: | Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200 F, 3001 Heverlee, Belgium. |
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Abstract: | In the present contribution, we demonstrated that surface-enhanced resonance Raman scattering spectra from single green fluorescent proteins (GFPs) were obtained. The most important findings are the direct detection of the conversion between a deprotonated and a protonated form of the chromophore at the single-molecule level via the corresponding vibrational fingerprints, and the fact that the enhanced green fluorescent protein (EGFP) also shows a high surface enhanced resonance Raman scattering (SERRS) signal. Our findings show the potential of the technique to study structural dynamics of protein molecules at a single-molecule level. |
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