| 毛细管区带电泳定量分析机体组织中高能磷酸化合物 |
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| 引用本文： | 慈薇,柴逸峰,刘荔荔,尹茶,吴玉田.毛细管区带电泳定量分析机体组织中高能磷酸化合物[J].分析化学,2001,29(10):1144-1146. |
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| 作者姓名： | 慈薇 柴逸峰 刘荔荔 尹茶 吴玉田 |
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| 作者单位： | 第二军医大学药学院药物分析教研室 |
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| 摘 要： | 研究了机体组织中三磷酸腺苷、二磷酸腺苷、一磷酸腺苷及磷酸肌酸的毛细管区带电泳分离条件；１２ｍｉｎ内完成上述４组分分离；其检出限分别为 ３．５、３．６、２．４、５．１ｍｇ／Ｌ；迁移时间及校正峰面积的定量精度分别低于０．１７％和３．８％。方法已用于猫心肌中上述４组分的定量分析。
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| 关 键 词： | 毛细管区带电泳 三磷酸腺苷 磷酸肌酸 猫心肌 |
| 修稿时间： | 2000年11月17 |
Quantitative Analysis of High-energy Phosphate Compounds in Body Tissue by Capillary Zone Electrophoresis |
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| Ci Wei,Chai Yifeng,Liu Lili,Yin Cha,Wu Yutian.Quantitative Analysis of High-energy Phosphate Compounds in Body Tissue by Capillary Zone Electrophoresis[J].Chinese Journal of Analytical Chemistry,2001,29(10):1144-1146. |
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| Authors: | Ci Wei Chai Yifeng Liu Lili Yin Cha Wu Yutian |
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| Abstract: | It is reported the application of capillary zone electrophoresis for the separation of adenosinetriphosphoric acid (ATP), adenosine-diphosphoric acid (ADP), adenosine-phosphoric acid (AMP) and creatine phosphate (CP). Under the chosen conditions, the analysis can be completed within 12 minutes. The concentration limits of detection (CLOD) for ATP, ADP, AMP and CP are 3.5 mg/L, 3.6 mg/L, 2.4 mg/L and 5.1 mg/L, respectively. The RSD of retention time and corrected peak area are 0.2% and 3.8%. The method has been used to the determination of above four compounds in cat heart. |
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| Keywords: | Capillary zone electrnphoresis adenosine-triphosphoric acid creatine phosphate cat heart |
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