Filterable Agents for Hyperpolarization of Water,Metabolites, and Proteins |
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| Authors: | Basile Vuichoud Dr. Aurélien Bornet Dr. Florian de Nanteuil Jonas Milani Estel Canet Xiao Ji Dr. Pascal Miéville Emmanuelle Weber Dr. Dennis Kurzbach Andrea Flamm Prof. Robert Konrat Dr. Alvar D. Gossert Dr. Sami Jannin Prof. Geoffrey Bodenhausen |
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| Affiliation: | 1. Institut des Sciences et Ingénierie Chimiques, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland;2. Département de Chimie, Ecole Normale Supérieure-PSL Research University, Paris, France;3. Sorbonne Universités, UPMC Univ Paris 06, Ecole Normale Supérieure, CNRS LBM, Paris, France;4. Institute of Biomolecular Structural Chemistry, University of Vienna, Vienna, Austria;5. Institutes for BioMedical Research, Novartis, Basel, Switzerland |
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| Abstract: | Hyperpolarization is generated by dissolution dynamic nuclear polarization (d‐DNP) using a polymer‐based polarizing agent dubbed FLAP (filterable labeled agents for polarization). It consists of a thermo‐responsive poly(N‐isopropylacrylamide), also known as pNiPAM‐COOH, labeled with nitroxide radicals. The polymer powder is impregnated with an arbitrary solution of interest and frozen as is. Dissolution is followed by a simple filtration, leading to hyperpolarized solutions free from any contaminants. We demonstrated the use of FLAP to hyperpolarize partially deuterated water up to P(1H)=6 % with a long relaxation T1 >36 s characteristic of high purity. Water hyperpolarization can be transferred to drugs, metabolites, or proteins that are waiting in an NMR spectrometer, either by exchange of labile protons or through intermolecular Overhauser effects. We also show that FLAPs are suitable polarizing agents for 13C‐labeled metabolites such as pyruvate, acetate, and alanine. |
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| Keywords: | dissolution-DNP filterable polarization agents hyperpolarization NMR spectroscopy thermo-responsive polymers |
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