首页 | 本学科首页   官方微博 | 高级检索  
     


NMR Spectroscopic Assignment of Backbone and Side‐Chain Protons in Fully Protonated Proteins: Microcrystals,Sedimented Assemblies,and Amyloid Fibrils
Authors:Dr. Jan Stanek  Dr. Loren B. Andreas  Dr. Kristaps Jaudzems  Dr. Diane Cala  Dr. Daniela Lalli  Andrea Bertarello  Dr. Tobias Schubeis  Dr. Inara Akopjana  Dr. Svetlana Kotelovica  Prof. Kaspars Tars  Dr. Andrea Pica  Dr. Serena Leone  Prof. Delia Picone  Dr. Zhi‐Qiang Xu  Prof. Nicholas E. Dixon  Dr. Denis Martinez  Mélanie Berbon  Nadia El Mammeri  Dr. Abdelmajid Noubhani  Dr. Sven Saupe  Dr. Birgit Habenstein  Dr. Antoine Loquet  Dr. Guido Pintacuda
Affiliation:1. Centre de RMN à Très Hauts Champs, Institut des Sciences Analytiques (UMR 5280 – CNRS, ENS Lyon, UCB Lyon 1), Université de Lyon, Villeurbanne, France;2. Biomedical Research and Study Centre, Riga, Latvia;3. Department of Chemical Sciences, University of Naples Federico II, Naples, Italy;4. School of Chemistry, University of Wollongong, NSW, Australia;5. Institute of Chemistry & Biology of Membranes & Nanoobjects (UMR 5248 CBMN – CNRS, University of Bordeaux, Bordeaux INP), All. Geoffroy Saint-Hillaire, Pessac, France;6. Institut de Biochimie et de Génétique Cellulaire (UMR 5095, CNRS –, Université de Bordeaux), Bordeaux, France
Abstract:We demonstrate sensitive detection of alpha protons of fully protonated proteins by solid‐state NMR spectroscopy with 100–111 kHz magic‐angle spinning (MAS). The excellent resolution in the Cα‐Hα plane is demonstrated for 5 proteins, including microcrystals, a sedimented complex, a capsid and amyloid fibrils. A set of 3D spectra based on a Cα–Hα detection block was developed and applied for the sequence‐specific backbone and aliphatic side‐chain resonance assignment using only 500 μg of sample. These developments accelerate structural studies of biomolecular assemblies available in submilligram quantities without the need of protein deuteration.
Keywords:magic-angle spinning  proton detection  resonance assignment  solid-state NMR spectroscopy
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号