Determinationation of kanamycin by square-wave cathodic adsorptive stripping voltammetry

A square-wave cathodic adsorptive stripping voltametric (SWCASV) method for the determination of kanamycin was developed on a thin-film mercury electrode (TFME). The optimal working conditions for the application of the method were found to be pH 8.0, provided by a Britton-Robinson (B.R.) buffer, and an adsorption potential of +0.30 V during 300 s. The equilibration time was applied during 10 s, and potential scans were performed at a scan rate of 40 mV/s, with a square-wave frequency of 100 Hz. The measuring-system response was linear over the kanamycin concentration range from 1.2 × 10⁻⁹ to 5.0 × 10⁻⁸ M and the detection limit achieved was 4.8 × 10⁻¹⁰ M. The relative error and relative standard deviation obtained were 1.20 and 4.67%, respectively. The voltammetric procedure was applied successfully to give a rapid and precise assay of kanamycin in kanamycin sulfate injection form. Published in Elektrokhimiya in Russian, 2008, Vol. 44, No. 12, pp. 1433–1437. The text was submitted by author in English