| Abstract: | Electrophoretic studies of pure proteins and mixtures of proteins have been made in an electrophoretic cell modified so that dilute samples are superimposed on more concentrated (or undiluted) samples of the same solution. We have found that when albumin or globulin is used alone at two concentrations in two cells as a descending electrophoretic system there are no complex boundaries observed. However, when both are used in conjunction with serum there is very definite reproducible evidence than an immobile “complex” is observed in the more concentrated solution. We can therefore postulate that there is a complex present in undiluted serum which is composed of albumin and one or more of the globulins cemented together by a heat stable dialyzable substance. This complex is immobile at pH 8.0. It has also been demonstrated that dilution and dialysis dissociate the complex. |
