Characteristics of a liposome immunoassay on a poly(methyl methacrylate) surface |
| |
| Authors: | Sang Youn Hwang Yoichi Kumada Gi Hoon Seong Jaebum Choo Shigeo Katoh Eun Kyu Lee |
| |
| Institution: | (1) Department of Chemical Engineering, Hanyang University, Ansan, South Korea;(2) Department of Chemistry and Materials Technology, Kyoto Institute of Technology, Kyoto, Japan;(3) Department of Applied Chemistry, Hanyang University, Ansan, South Korea;(4) Department of Chemical Science and Engineering, Kobe University, Kobe, Japan |
| |
| Abstract: | Liposome immunoassay (LIA) is based on enzyme immunoassay (EIA) but the detection sensitivity could be significantly enhanced
by using antibody-coupled immunoliposomes encapsulating HRP (horse radish peroxidase). Here, we applied LIA to non-porous
poly(methyl methacrylate) (PMMA) and polystyrene (PS) surfaces to compare its detection sensitivity with that of EIA, using
rabbit IgG (a ligand molecule) and anti-rabbit IgG antibody (a capture molecule) as the model system. LIA developed much stronger
color signals than EIA, especially at a lower concentration range (< ca. 1 μg mL−1). PMMA showed higher affinity toward rabbit IgG than the PS surface, and the anti-rabbit IgG antibody adsorbed on PMMA was
more stable than that on PS. Furthermore, the effects of spot volume and antibody concentration on the signal density were
analyzed. The signal density increased as the antibody concentration increased, but it was not significantly affected by the
spot volume (2.5–20 μL). In conclusion, LIA on PMMA as a solid support is a very useful, highly sensitive microarray detection
system.
Sang Youn Hwang and Yoichi Kumada have same rights on this paper. |
| |
| Keywords: | Liposome immunoassay Immunoliposome PMMA Microarray detection Signal enhancement |
| 本文献已被 PubMed SpringerLink 等数据库收录！ |
|
