Noncovalent labeling of biomolecules with red and near- infrared dyes |
| |
Authors: | Patonay Gabor Salon Jozef Sowell John Strekowski Lucjan |
| |
Institution: | Department of Chemistry, Georgia State University, University Plaza, Atlanta, Georgia 30303, USA. cheggp@panther.gsu.edu |
| |
Abstract: | Biopolymers such as proteins and nucleic acids can be labeled with a fluorescent marker to allow for their detection. Covalent labeling is achieved by the reaction of an appropriately functionalized dye marker with a reactive group on a biomolecule. The recent trend, however, is the use of noncovalent labeling that results from strong hydrophobic and/or ionic interactions between the marker and biomolecule of interest. The main advantage of noncovalent labeling is that it affects the functional activity of the biomolecule to a lesser extent. The applications of luminescent cyanine and squarylium dyes are reviewed. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|