An Indirect Competitive Enzyme-linked Immunosorbent Assay for Simultaneous Determination of Florfenicol and Thiamphenicol in Animal Meat and Urine |
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Authors: | Ran LI Ze-Jia LIN Jin-Yi YANG Zhen-Lin XU Hong WANG Hong-Tao LEI Yuan-Ming SUN Yu-Dong SHEN |
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Affiliation: | Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China |
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Abstract: | A high-affinity polyclonal antibody was prepared by immunizing animals with haptens FFD and FFM. Under the optimal combination of coating antigen and antibody, an indirect competitive enzyme-linked immunosorbent assay (icELISA) for simultaneous detection of florfenicol and thiamphenicol residues in animal meat and urine samples was developed. The icELISA showed an IC50 value of 1.32 ng mL?1 for florfenicol and 2.13 ng mL?1 for thiamphenicol, respectively. The linear ranges were from 0.31 to 5.61 ng mL?1 with a limit of detection of 0.12 ng mL?1 for florfenicol, and 0.41 to 11.2 ng mL?1 with a limit of detection of 0.15 ng mL?1 for thiamphenicol, respectively. The average recoveries of florfenicol and thiamphenicol in spiked samples ranged from 77.2% to 116.0% with a relative standard deviation of less than 15%. Therefore, this proposed icELISA provided a valid detection method for florfenicol and thiamphenicol residues in animal tissue and urine samples. |
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Keywords: | Florfenicol Thiamphenicol Antibody Indirect competitive enzyme-linked immunosorbent assay |
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