| 利用噬菌体展示技术制备识别酵母RNR_3原核表达片段的单链抗体 |
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| 引用本文： | 李佳,宋波,王玉珍,饶瑜,戴和平.利用噬菌体展示技术制备识别酵母RNR_3原核表达片段的单链抗体[J].武汉大学学报(理学版),2009,55(5). |
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| 作者姓名： | 李佳 宋波 王玉珍 饶瑜 戴和平 |
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| 作者单位： | 1. 中国科学院,水生生物研究所,湖北,武汉,430072;中国科学院研究生院,北京,100049
2. 中国科学院,水生生物研究所,湖北,武汉,430072 |
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| 摘 要： | 选取酵母细胞RNR_3蛋白序列中高保守片段:145-298位氨基酸序列,命名为RNR_3h进行原核表达.并利用噬菌体展示技术从非免疫小鼠噬菌体展示抗体库中淘选到能与该原核表达产物特异性结合的单链抗体.得到的3种单链抗体均识别原核表达片段的空间抗原位点.为寻找酵母RNR1和RNR_3的诱导表达水平与DNA损伤信号的相互关系,将真核生物RNRs发展为检测环境化学致痛物DNA损伤效应的生物标志物进行了尝试.
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| 关 键 词： | 核糖核苷酸还原酶 酵母RNR_3 噬菌体展示技术 单链抗体 |
Preparation scFvs against the Procaryotic Truncated Fragment of RNR_3 in an Unimmuned Antibody Phage Display Library |
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| LI Jia,SONG Bo,WANG Yuzhen,RAO Yu,DAI Heping.Preparation scFvs against the Procaryotic Truncated Fragment of RNR_3 in an Unimmuned Antibody Phage Display Library[J].JOurnal of Wuhan University:Natural Science Edition,2009,55(5). |
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| Authors: | LI Jia SONG Bo WANG Yuzhen RAO Yu DAI Heping |
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| Abstract: | In this study,a selected homologous peptide from Saccharomyces cerevisiae RNR3 (locl45-loc298) named RNR3h was expressed in E.coli,and the purified protein is used as an antigen to prepare specifically binding scFvs (single chain Fragment-variable) from an unimmuned mouse sourced antibody phage display library.Three different scFvs (single chain Fragment-variable region) that recognize the spatial epitope of RNR3h were obtained and further analyzed.Preliminary studies were made to discover the RNR1 and RNR3's expression features that induced by DNA damage,and to develop RNR3 as a bio-marker to detect the chemical carcinogens in environment by reacting to DNA damage. |
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| Keywords: | RNR RNR3 phage display technology single chain Fragment-variable(scFvs) |
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