Reconstitution of a new cysteine biosynthetic pathway in Mycobacterium tuberculosis |
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Authors: | Burns Kristin E Baumgart Sabine Dorrestein Pieter C Zhai Huili McLafferty Fred W Begley Tadhg P |
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Institution: | Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853, USA. |
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Abstract: | A new pathway for cysteine biosynthesis has been elucidated in Mycobacterium tuberculosis. This pathway involves a protein-bound thiocarboxylate (CysO-SH) as the sulfide donor, similar to thiamin biosynthesis. Cysteine synthase M (CysM) catalyzes the addition of cysteine to the carboxy terminus of the protein-bound thiocarboxylate to generate a CysO-cysteine adduct. A protease, Mec+, hydrolyzes the CysO-cysteine adduct to release cysteine and regenerate CysO. Mec+ contains a JAMM motif, and this work provides the first functional characterization of the JAMM motif in prokaryotes. MoeZ, a paralogue of ThiF, has been shown to transfer sulfur onto CysO. |
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