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Effect of Regulation of HSV-tk Gene Expression and Tumor Killed Activity with a Single Tetracycline-regulatable Plasmid Vector on HeLa Cells |
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| Authors: | WANG Qian DU Zhen-wu MA Qing-shan ZHANG Yu-cheng WU Xiao-dong YANG Shao-juan WANG Ya-li ZHANG Gui-zhen . Central Laboratory |
| Affiliation: | [1]Central Laboratory,China-Japan Union Hospital, Jilin University, Changchun 130033, P. R. China, [2]Department of Urinary Surgery, China-Japan Union Hospital, Jilin University, Changchun 130033, P. R. China [3]School of Pharmaceutical Sciences,The First Hospital, Jilin University, Changehun 130021, P. R. China [4]Department of Pediatrics, The First Hospital, Jilin University, Changehun 130021, P. R. China |
| Abstract: | To construct a single tetracycline-regulatable plasmid vector based on the double tetracycline-regulatable plasmid vector system for regulating HSV-tk gene expression so as to effectively kill HeLa cells. Two tetracycline operator(TetO2) was cloned into pcDNA3.1 and a cassette was made for a cytomegalovirus-type 2 tetracycline operator(CMV-TetO2) promoter, and the obtained vector was named pcDNA3.1-CMV-TetO2. Herpes simplex virus thymidine kinase(HSV-tk) gene and tetracycline repressor(TR) gene were cloned ... |
| Keywords: | HSV-tk gene Tetracycline HeLa cells Gene expression |
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