Development of a low-cost microfluidic capillary-electrophoresis system coupled with flow-injection and sequential-injection sample introduction (review) |
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Authors: | Z-L Fang Qun Fang |
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Institution: | (1) Zhejiang University, Chemistry Department, Institute of Microanalytical Systems, Xixi Campus, 310028 Hangzhou, China e-mail: fangzl@mail.sy.ln.cn, CN;(2) Northeastern University, Chemistry Department, Research Center for Analytical Sciences, 110006 Shenyang, China, CN |
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Abstract: | Microfabrication techniques used for the production of MEMS (micro electro-mechanical systems) have been successfully used
to produce highly efficient microfluidic capillary electrophoresis chip systems. A limitation of this approach are the difficulties
associated with the creation of the micrometer-sized structures in glass or other substrates, which currently involve specialized
and expensive lithographic and etching processes. A further limitation is that hitherto most microfluidic chips are not designed
for continuous introduction of a series of different samples, which limits the overall throughput of such systems. This article
reviews the development of a microfluidic system for rapid CE separations, produced at a low cost of less than a dollar each,
using equipment and materials readily available in the ordinary laboratory. Applications of the system, after coupling to
flow-injection and/or sequential-injection sample introduction, for the determination of FITC- labeled amino acids by laser-induced
fluorescence, trace metals by chemiluminescence, carbohydrates by amperometry, and inorganic and organic anions by indirect
UV absorbance are exemplified to illustrate the performance and versatility of the microfluidic system.
Received: 30 November 2000 / Revised: 13 February 2001 / Accepted: 23 February 2001 |
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