| 双光子和多光子共焦显微镜的成像理论 |
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| 引用本文： | 唐志列,梁瑞生,常鸿森.双光子和多光子共焦显微镜的成像理论[J].物理学报,2000,49(6):1076-1080. |
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| 作者姓名： | 唐志列 梁瑞生 常鸿森 |
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| 作者单位： | 华南师范大学物理系，广州 510631 |
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| 基金项目： | 广东省自然科学基金(批准号：980046)和广东省科委“九·五”重点攻关项目资助的课题. |
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| 摘 要： | 对双光子和多光子共焦扫描显微镜的成像理论作了系统的理论分析，导出了双光子和多光子共焦显微镜成像系统的三维点扩散函数和三维光学传递函数，研究结果表明：双光子共焦显微镜比单光子共焦显微镜具有更高的横向分辨率和纵向分辨率，而多光子共焦扫描显微镜又比双光子共焦扫描显微镜具有更高的空间分辨率.
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| 关 键 词： | 双光子 多光子 共焦显微镜 成像理论 |
| 收稿时间： | 1999-10-13 |
THE IMAGING THEORY OF TWO-PHOTON AND MULTI-PHOTON CONFOCAL SCANNING MICROSCOPY |
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| TANG ZHI-LIE,LIANG RUI-SHENG and CHANG HONG-SEN.THE IMAGING THEORY OF TWO-PHOTON AND MULTI-PHOTON CONFOCAL SCANNING MICROSCOPY[J].Acta Physica Sinica,2000,49(6):1076-1080. |
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| Authors: | TANG ZHI-LIE LIANG RUI-SHENG and CHANG HONG-SEN |
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| Abstract: | The Imaging theory of two-photon and multi-photon confocal scanning microscopy is presented. The three-dimensional point spread function and three-dimensional optical transfer function of two-photon confocal scanning microscopy is derived. By using the three-dimensional point spread function and three-dimensional optical transfer function theory, we have proved that the horizontal and vertical resolutions of two-photon confocal scanning microscopy are higher than those of the single photon confocal scanning microscopy, and the space resolution of multi-photon confocal scanning microscopy is higher than that of two-photon confocal scanning microscopy. |
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