A liquid chromatography-mass spectrometry method for the quantitation of aristololactam-I in rat plasma |
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Authors: | Ling Zhen Jiang Zhen-zhou Huang Xin Zhang Lu-yong Xu Xiao-yue |
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Institution: | New Drug Screen Centre, China Pharmaceutical University, Nanjing, 210038, People's Republic of China. |
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Abstract: | A sensitive method with liquid chromatography-electrospray ionization mass spectrometry has been developed and validated for the determination of aristololactam-I in rat plasma after oral administration of aristolochic acid-I using finesteride as the internal standard. Chromatographic separation was achieved on a Lichrospher C(18) column using methanol:0.05% acetic acid in water (71:29, v/v) as a mobile phase delivered at a flow rate of 1 mL/min. The assay was linear for aristololactam-I over the range 0.3-300 ng/mL. The analysis of quality control samples demonstrated precision with coefficient of variation less than 20% (n = 5). Absolute recovery of aristololactam-I was 90.4-97.3%. The LC-MS method for the determination of aristololactam-I is sensitive, specific and can be used to investigate the toxicokinetics of aristololactam-I. |
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Keywords: | aristololactam‐I aristolochic acid‐I rat plasma LC‐MS toxicokinetics |
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