| 紫外光谱法对维生素E油酸酯、维生素E与油酸的同时测定 |
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| 引用本文： | 粟晖,黎国梁,姚志湘,郭晓菲. 紫外光谱法对维生素E油酸酯、维生素E与油酸的同时测定[J]. 分析测试学报, 2009, 28(11). DOI: 10.3969/j.issn.1004-4957.2009.11.019 |
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| 作者姓名： | 粟晖 黎国梁 姚志湘 郭晓菲 |
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| 作者单位： | 广西工学院,生物与化学工程系,广西,柳州,545006;广西大学,化学化工学院,广西,南宁,530004 |
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| 基金项目： | 广西自然科学基金资助项目，广西教育厅桂教科研资助项目 |
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| 摘 要： | 建立了混合体系中维生素E、油酸和维生素E油酸酯同时测定的方法,用光纤光谱仪获取混合体系紫外-可见透射光谱.实验按均匀设计建立校正集和预测集,在255 ～315 nm波段采用偏最小二乘法建立了同时定量测定该3组分的校正模型,并用间隔区间偏最小二乘法(iPLS)通过优选建模区间改进油酸的预测模型.采用iPLS能够显著提高模型准确度,尤其对光谱弱响应的物质,最大相对误差从PLS直接建模的54.7%降至iPLS的8.98%,建立的模型可满足动力学研究的原位分析需要.
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| 关 键 词： | 原位分析 VE-油酸-VE油酸酯 偏最小二乘法 间隔区间偏最小二乘法 |
Simultaneous Determination of Vitamin E Oleate,Vitamin E and Oleic Acid by Ultraviolet Spectroscopy Method |
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| Abstract: | A simultaneous determination method was established for the determination of vitamin E, oleic acid and vitamin E oleate. The mixture signal was gotten by UV - Vis fiber-optical spectrometer. In this experiment, calibration set and prediction set were designed by uniform design. A calibration model of three components system for simultaneous determination was built by the partial least square at 255 - 315 nm and a predicted model of oleic acid was improved by interval PLS algorithm at selected optimal interval. The results showed that, comparing with the outputs of PLS, iPLS could apparently improve the accuracy of prediction, especially for components with low absorbance signal, and the maximum relative error reduced from 54. 7% to 8. 98% . Therefore, the constructed model was suitable for the requirement of in-situ anlysis for dynamic study. |
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| Keywords: | in-situ analysis VE - oleic acid - vitamin E oleate partial least squres interval partial least squares |
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