Effect of cations on the structure of bilayers formed by lipopolysaccharides isolated from Pseudomonas aeruginosa PAO1 |
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Authors: | Kucerka Norbert Papp-Szabo Erzsebet Nieh Mu-Ping Harroun Thad A Schooling Sarah R Pencer Jeremy Nicholson Eric A Beveridge Terry J Katsaras John |
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Affiliation: | Canadian Neutron Beam Centre, National Research Council, Chalk River, Ontario K0J 1J0, Canada. |
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Abstract: | The asymmetric outer membrane of Gram-negative bacteria contains lipopolysaccharides (LPSs) which contribute significantly to the bacterium's surface properties and play a crucial role in regulating membrane permeability. We report on neutron diffraction studies performed on aligned, self-assembled bilayers of Na-, Ca-, and Mg-salt forms of LPS isolated from Pseudomonas aeruginosa PAO1. From the one-dimensional neutron scattering length density profiles we find that water penetrates Ca2+-LPS bilayers to a lesser extent than either Na+- or Mg2+-LPS bilayers. This differential water penetration could have implications as to how small molecules permeate the outer membrane of Gram-negative bacteria and, possibly, how nonlamellar phases are formed. |
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