Highly branched stimuli responsive poly[(N-isopropyl acrylamide)-co-(1,2-propandiol-3-methacrylate)]s with protein binding functionality |
| |
Authors: | Carter Steven Rimmer Stephen Sturdy Alice Webb Michelle |
| |
Institution: | The Polymer and Biomaterials Chemistry Laboratories, Department of Chemistry (Polymer Centre), Brook Hill, University of Sheffield, Sheffield, South Yorkshire, UK S3 7HF, United Kingdom. |
| |
Abstract: | Highly branched poly(NIPAM) have been prepared using the technique of reversible addition-fragmentation chain transfer (RAFT) polymerisation using a chain transfer agent that allows the incorporation of imidazole functionality in the polymer chain-ends. The lower critical solution temperature (LCST) of the polymers can be controlled by the amount of hydrophobe and GMA incorporated during copolymerisation procedures. These thermally responsive "smart" polymers were used to purify a His-tagged BRCA-1 protein fragment by affinity precipitation. Diagram: see text] |
| |
Keywords: | affinity precipitation copolymerization His‐tagged protein reversible addition‐fragmentation chain transfer (RAFT) water‐soluble polymers |
本文献已被 PubMed 等数据库收录! |