| VISUALIZATION OF DYNAMIC ORGANIZATION OF CYTOSKELETON GELS IN LIVING CELLS BY HYBRID-SPM |
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| 引用本文： | K.Kawabata,Y.Sado,M.Nagayama,T.Nitta,K.Nemoto,Y.Koyama,H.Haga. VISUALIZATION OF DYNAMIC ORGANIZATION OF CYTOSKELETON GELS IN LIVING CELLS BY HYBRID-SPM[J]. 高分子科学, 2003, 0(2): 169-174 |
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| 作者姓名： | K.Kawabata Y.Sado M.Nagayama T.Nitta K.Nemoto Y.Koyama H.Haga |
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| 作者单位： | Divisions of Biological Sciences Graduate School of Science，Hokkaido University，060-0810 Sapporo，Divisions of Physics，Graduate School of Science. Hokkaido University，060-0810 Sapporo，Divisions of Physics，Graduate School of Science. Hokkaido University，060-0810 Sapporo，Divisions of Physics，Graduate School of Science. Hokkaido University，060-0810 Sapporo，Divisions of Physics，Graduate School of Science. Hokkaido University，060-0810 Sapporo，Department of Biochemistry，Hokkaido University Graduate School of Medicine，Sapporo 060-8638，Japan，Divisions of Physics，Graduate School of Science，Hokkaido University，060-0810 Sapporo |
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| 基金项目： | This work is partially supported by the Special Funding for Basic Research, Ministry of Education, Science, Sports and Culture, Japan, to K. K. |
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| 摘 要： | We succeeded in performing of hybrid Scanning Probe Microscopy (hybrid-SPM) in which mechanical-SPM andfluorescence microscopy are combined. This technique is able to measure simultaneously mechanical properties anddistribution of cytoskeletons of living cells by using green fluorescent protein. We measured evolution of both local elasticityand distributions of actin stress fibers in an identical fibroblast living in physiological conditions. The SPM experimentsrevealed that stiffer lines develop in living cells, which correspond to actin stress fibers. The elasticity of the actin stressfibers is as high as 100 kPa. We discuss mechanical effects on the development of actin filament networks.
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| 收稿时间： | 2002-08-31 |
VISUALIZATION OF DYNAMIC ORGANIZATION OF CYTOSKELETON GELS IN LIVING CELLS BY HYBRID-SPM* |
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| K. Kawabata,Y. Sado,M. Nagayama,T. Nitta,K. Nemoto,Y. Koyama,H. Haga. VISUALIZATION OF DYNAMIC ORGANIZATION OF CYTOSKELETON GELS IN LIVING CELLS BY HYBRID-SPM*[J]. Chinese Journal of Polymer Science, 2003, 0(2): 169-174 |
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| Authors: | K. Kawabata Y. Sado M. Nagayama T. Nitta K. Nemoto Y. Koyama H. Haga |
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| Affiliation: | a.Divisions of Biological Sciences and physics; Graduate School of Science. Hokkaido University; 060-0810 Sapporo; b.Department of Biochemistry Hokkaido University Graduate School of Medicine; Saporo 060-8638; Japan |
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| Abstract: | We succeeded in performing of hybrid Scanning Probe Microscopy (hybrid-SPM) in which mechanical-SPM andfluorescence microscopy are combined. This technique is able to measure simultaneously mechanical properties anddistribution of cytoskeletons of living cells by using green fluorescent protein. We measured evolution of both local elasticityand distributions of actin stress fibers in an identical fibroblast living in physiological conditions. The SPM experimentsrevealed that stiffer lines develop in living cells, which correspond to actin stress fibers. The elasticity of the actin stressfibers is as high as 100 kPa. We discuss mechanical effects on the development of actin filament networks. |
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| Keywords: | Scanning probe microscopy Green fluorescent protein Fibroblast Cell migration Cytoskeleton Elasticity |
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