Separation and tentative identification of the main pigment fraction of raisins by thin-layer chromatography-Fourier transform infrared and high-performance liquid chromatography-ultraviolet detection |
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Authors: | Cserháti T Forgács E Candeias M Vilas-Boas L Bronze R Spranger I |
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Institution: | Institute of Chemistry, Chemical Research Centre, Hungarian Academy of Sciences, Budapest. |
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Abstract: | The soluble color pigments of raisin are separated by reversed-phase thin-layer chromatography (TLC), and the capacity of TLC-Fourier transform infrared (FTIR) with both on-line and off-line coupling is assessed for the identification of the main fraction. TLC has also been used as a pilot technique for the development of a gradient elution method for the separation of pigments by high-performance liquid chromatography (HPLC). On-line TLC-FTIR cannot be used for identification because of the strong adsorbance of the stationary phase. Off-line TLC-FTIR combined with the retention behavior of the main pigment fraction indicates that it is a polymer, caramel-like compound composed of erythrose and fructose monomers. Baseline separation of pigments is achieved by HPLC using TLC as a pilot method. |
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