| 血卟啉衍生物与血红蛋白相互作用的光谱特性研究 |
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| 引用本文： | 冯尚源,陈荣,杨文琴,李永增,黄祖芳,廖小华. 血卟啉衍生物与血红蛋白相互作用的光谱特性研究[J]. 光谱学与光谱分析, 2007, 27(11): 2279-2282 |
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| 作者姓名： | 冯尚源 陈荣 杨文琴 李永增 黄祖芳 廖小华 |
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| 作者单位： | 福建师范大学化学与材料学院实验中心,福建,福州,350007;福建师范大学医学光电科学与技术教育部重点实验室,福建,福州,350007;福建师范大学医学光电科学与技术教育部重点实验室,福建,福州,350007;福建师范大学化学与材料学院实验中心,福建,福州,350007 |
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| 基金项目： | 教育部科学技术研究项目 , 福建省科技厅科技计划 , 福建省教育厅科研项目 |
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| 摘 要： | 采用荧光光谱法和紫外-可见吸收光谱法研究了在不同温度下，血卟啉衍生物(HPD)与血红蛋白(Hb)结合反应的荧光猝灭光谱行为。证实由于非辐射能量转换机制，HPD对Hb有较强的荧光猝灭能力。根据Stern-Volmer方程和Lineweaver-Burk双倒数函数曲线，计算其结合常数在高温时要比低温时大，并获得相关结合常量和热力学参数。根据Fster能量传递原理计算出临界结合位点R0＝3.22 nm。
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| 关 键 词： | 血卟啉衍生物(HPD) 血红蛋白(Hb) 荧光光谱 猝灭 |
| 文章编号： | 1000-0593（2007）11-2279-04 |
| 收稿时间： | 2006-08-16 |
| 修稿时间： | 2006-11-18 |
Spectral Properties of the Hemoporphyrin Derivative Interacting with Hemoglobin |
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| FENG Shang-yuan,CHEN Rong,YANG Wen-qin,LI Yong-zeng,HUANG Zu-fang,LIAO Xiao-hua. Spectral Properties of the Hemoporphyrin Derivative Interacting with Hemoglobin[J]. Spectroscopy and Spectral Analysis, 2007, 27(11): 2279-2282 |
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| Authors: | FENG Shang-yuan CHEN Rong YANG Wen-qin LI Yong-zeng HUANG Zu-fang LIAO Xiao-hua |
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| Affiliation: | 1. College of Chemistry and Material Science，Experimental Center, Fujian Normal University，Fuzhou 350007，China2. Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education，Fujian Normal University，Fuzhou 350007，China |
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| Abstract: | The quenching reaction of hemoporphyrin derivative with hemoglobin (Hb) was studied by using fluorescence spectra and absorption spectra.It was shown that HpD has a powerful ability to quench the Hb fluorescence via a nonradiative energy transfer mechanism. The formation constants of them were analyzed at different temperature according to Stern-Volmer equation and double-reciprocal equation, which are bigger at high temperature than at low temperature. The critical binding site was calculated (R0=3.22 nm) by Fster energy transfer mechanism, and the thermodynamic parameters were obtained. |
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| Keywords: | Hemoglobin Hemoporphyrin derivative Fluorescence spectra Quenching |
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