| 检测禽流感H5亚型病毒的阻抗型免疫研究 |
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| 引用本文： | 颜小飞,李运涛,王蓉晖,林建涵,温新华,汪懋华,安冬,韩伟静,俞育德.检测禽流感H5亚型病毒的阻抗型免疫研究[J].分析化学,2012(10):1507-1513. |
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| 作者姓名： | 颜小飞 李运涛 王蓉晖 林建涵 温新华 汪懋华 安冬 韩伟静 俞育德 |
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| 作者单位： | 中国农业大学教育部现代精细农业系统集成研究重点实验室;中国科学院半导体所集成光电子学国家重点实验室;Department of Biological and Agricultural Engineering,University of Arkansas |
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| 基金项目： | 国际科技交流与合作专项(No.2010DFA31000)资助 |
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| 摘 要： | 研制了一种可用于H5亚型禽流感病毒快速检测的阻抗型免疫传感器。通过蛋白A将H5N1表面抗原血凝素(HA)的单克隆抗体固定于金叉指阵列微电极表面,并与待测溶液中的目标抗原H5N1进行免疫反应。在Fe(CN)6]3"/4"溶液中进行电化学阻抗谱扫描,表征电极的表面修饰及抗原捕获过程。当H5N1病毒浓度在21～26 HA unit/50μL范围时,其浓度的对数值与叉指阵列微电极的电子传递阻抗的变化值呈线性关系,相关系数为0.9885;检出限为20 HA unit/50μL,检测时间为1 h。此传感器特异性好,灵敏度高,可以重复使用,在病原微生物快速检测领域具有良好的应用前景。
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| 关 键 词： | 阻抗型免疫传感器 禽流感病毒 电化学阻抗谱 叉指阵列微电极 |
An Impedance Immunosensor for Detection of H5 Subtype Avian Influenza Virus |
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| YAN Xiao-Fei,LI Yun-Tao,WANG Rong-Hui,LIN Jian-Han,WEN Xin-Hua,WANG Mao-Hua,AN Dong,HAN Wei-Jing,YU Yu-De.An Impedance Immunosensor for Detection of H5 Subtype Avian Influenza Virus[J].Chinese Journal of Analytical Chemistry,2012(10):1507-1513. |
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| Authors: | YAN Xiao-Fei LI Yun-Tao WANG Rong-Hui LIN Jian-Han WEN Xin-Hua WANG Mao-Hua AN Dong HAN Wei-Jing YU Yu-De |
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| Institution: | 2,LI Yan-Bin3 1(Key Laboratory on Modern Precision Agriculture System Integration Research, Ministry of Education,China Agricultural University,Beijing 100083,China) 2(State Key Laboratory of Integrated Optoelectronics,Institute of Semiconductor, Chinese Academy of Sciences,Beijing 100083,China) 3(Department of Biological and Agricultural Engineering,University of Arkansas,Fayetteville,AR 72701,USA) |
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| Abstract: | An impedance immunosensor was developed for the rapid detection of H5 subtype avian influenza virus(AIV).Monoclonal antibodies against AIV H5N1 surface antigen hemagglutinin(HA) were immobilized on the surface of gold interdigitated array microelectrodes through protein A for capturing AIV H5N1 in sample solutions.Electrochemical impedance spectroscopy in the presence of Fe(CN)6]3-/4- as a redox probe was used to describe the surface modification of microelectrodes and the binding of viruses.A linear relationship between the logarithmic value of concentration of AIV H5N1 and the change of electron transfer resistance was found in the concentration range of 21-26 HA unit per 50 μL,and its correlation coefficient was 0.9885.The detection limit was 20 HA unit per 50 μL,and the detection time was 1 h.This immunosensor could be used repeatedly with good specificity and high sensitivity,and it is promising for rapid detection of pathogenic microorganisms. |
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| Keywords: | Impedance immunosensor Avian influenza virus Electrochemical impedance spectroscopy Interdigitated array microelectrodes |
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