十二烷基苯磺酸钠-异辛烷-正辛醇反胶束萃取苦参生物碱的研究

利用阴离子表面活性剂十二烷基苯磺酸钠(SDBS)与溶剂异辛烷和助溶剂正辛醇形成反胶束体系,用于苦参生物碱的萃取分离。研究了pH值、表面活性剂浓度、增溶水量W0、盐种类及浓度等因素对萃取的影响。结果表明:SDBS-异辛烷-正辛醇反胶束体系对苦参生物碱具有良好的选择性和较高萃取率,在pH 5.0,增溶水量W0 25,0.05 mol/L SDBS,0.05 mol/L KCl,室温,萃取时间5 min,反萃取时间20 min的最佳萃取条件下,氧化苦参碱、氧化槐果碱、槐定碱、苦参碱、槐果碱5种生物碱及总生物碱的萃取率和RSD分别在74.1%～87.2%和0.63%～3.0%之间。本方法选择性高,操作简便。

Extraction of Alkaloids in Sophora FlavescensAit by Reverse Micelle of Sodium Dodecyl Benzene Sulfonate/Isooctane/n-Octanol

A method of reverse micelle extraction of alkaloids in Sophora Flavescens Ait has been developed using the reverse micelle system of sodium dodecyl benzene sulfonate(SDBS)/isooctane/n-octanol.The experimental conditions such as pH,water content W0,extraction time and temperature,the concentration of surfactant and salts were optimized.With the help of high performance liquid chromatography,the results showed that the reverse micelle formed by SDBS,isooctane and n-octanol can more effectively extract alkaloids than others.The optimum extraction conditions were as follows: pH 5.0,25 of water content W0,0.05 mol/L of SDBS concentration,0.05 mol/L of KCl concentration,5 min of forward-extraction time and 20 min of backward-extraction time at room temperature.The method has been applied to separate and purify alkaloids from the extract by water with pressurized microwave-assisted extraction.Under the optimum conditions,the extraction yields of oxymatrine,n-oxysophocarpine,sophoridine,matrine,sophocarpine and total alkaloids in Sophora Flavescens Ait were between 74.1% and 87.2%,RSDs of their reproducibility were from 0.6% to 3.0%.The method is higher selectivity,and easy to operate.