Optimization of substrate conversion in hollow fiber reactor

The crude jackbean preparation was used as urease to determine the Km_(app), V_(app), and substrate conversion (%) at different flow rates in a hollow fiber device. It was found that, as the flow rate increases, the V_(app) increases, whereas the Km_(app) initially decreases and again increases. The maximum substrate conversion (96.25%) was obtained at the lowest Km_(app). No significant loss was observed in substrate conversion after 2 mo at room temperature.