| 液相色谱/电喷雾质谱(LC/ESI/MS)在蛋白质分析鉴定中的应用 |
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| 引用本文： | 郭寅龙,刘晗青,余翀天.液相色谱/电喷雾质谱(LC/ESI/MS)在蛋白质分析鉴定中的应用[J].分析测试技术与仪器,2001,7(3):129-133. |
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| 作者姓名： | 郭寅龙 刘晗青 余翀天 |
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| 作者单位： | 中国科学院上海有机化学研究所,上海,200032 |
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| 基金项目： | 上海市自然科学基金资助项目 ( 0 0ZA14 0 77) |
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| 摘 要： | 对液相色谱／电喷雾质谱（LC／ESI／MS）在蛋白质分子量测定、一级结构的分析、蛋白质和多肽纯度的鉴定、肽质量酶谱、蛋白质分子内二硫键的定量和定位、磷酰化位置的测定以及在蛋白质组中的应用等方面进行了综述和讨论。
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| 关 键 词： | 液相色谱 电喷雾质谱 肽质量指纹图谱法 分子内二硫键 分析 鉴定 糖基化位置 磷酸化位置 蛋白质 |
| 文章编号： | 1006-3757(2001)03-0129-05 |
| 收稿时间： | 2001/3/20 0:00:00 |
| 修稿时间： | 2001年3月20日 |
The Applications of Liquid Chromatography-Electrospray Ionization Mass Spectrometry in Analysis and Identifications of Proteins |
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| GUO Yin-long,LIU Han-qing and YU Chong-tian.The Applications of Liquid Chromatography-Electrospray Ionization Mass Spectrometry in Analysis and Identifications of Proteins[J].Analysis and Testing Technology and Instruments,2001,7(3):129-133. |
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| Authors: | GUO Yin-long LIU Han-qing and YU Chong-tian |
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| Institution: | Shanghai Institute of Organic Chemistry, the Chinese Academy of Sciences, Shanghai 200032, China;Shanghai Institute of Organic Chemistry, the Chinese Academy of Sciences, Shanghai 200032, China;Shanghai Institute of Organic Chemistry, the Chinese Academy of Sciences, Shanghai 200032, China |
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| Abstract: | A review with 32 references is focusing on determination of molecular weight (MW) of proteins, analysis for primary structure of proteins and peptides and identification of purification of proteins and peptides by liquid chromatography electrospray ionization mass spectrometry. Applications in peptide mass mapping, quantification and determination of positions of disulfide bridges of proteins and peptides, determination of positions of phosphate and applications in proteins, et al were discussed. |
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| Keywords: | liquid chromatography electrospray ionization mass spectrometry peptide mass mapping disulfide bridges glycosylation sites phosphorylation sites proteome |
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