Oxidized Xanthan Gum Crosslinked NOCC: Hydrogel System and Their Biological Stability from Oxidation Levels of the Polymer |
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Authors: | Vo Minh Quan Dat-Quoc Do Tin Dai Luong Tuan-Ngan Tang Binh Thanh Vu Hien-Phuong Le Phuc H. Vo Nhi Ngoc-Thao Dang Quyen Ngoc Tran Nhu-Thuy Trinh Thi-Hiep Nguyen |
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Affiliation: | 1. School of Biomedical Engineering, International University, Ho Chi Minh, 70000 Vietnam;2. School of Biomedical Engineering, International University, Ho Chi Minh, 70000 Vietnam Vietnam National University, Ho Chi Minh, 70000 Vietnam;3. Institute of Applied Materials Science, Vietnam Academy Science and Technology, Ho Chi Minh, 70000 Vietnam Graduate University of Science and Technology Viet Nam, Vietnam Academy of Science and Technology, Ho Chi Minh, 70000 Vietnam |
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Abstract: | Dynamic hydrogel systems from N,O-carboxymethyl chitosan (NOCC) are investigated in the past years, which has facilitated their widespread use in many biomedical engineering applications. However, the influence of the polymer's oxidation levels on the hydrogel biological properties is not fully investigated. In this study, chitosan is converted into NOCC and introduced to react spontaneously with oxidized xanthan gum (OXG) to form several injectable hydrogels with controlled degradability. Different oxidation levels of xanthan gum, as well as NOCC/OXG volume ratios, are trialed. The infrared spectroscopy spectra verify chemical modification on OXG and successful crosslinking. With increasing oxidation levels, more dialdehyde groups are introduced into the OXG, resulting in changes in physical properties including gelation, swelling, and self-healing efficiency. Under different volume ratios, the hydrogel shows a stable structure and rigidity with higher mechanical properties, and a slower degradation rate. The shear-thinning and self-healing properties of the hydrogels are confirmed. In vitro assays with L929 cells show the biocompatibility of all formulations although the use of a high amount of OXG15 and OXG25 limited the cell proliferation capacity. Findings in this study suggested a suitable amount of OXG at different oxidation levels in NOCC hydrogel systems for tissue engineering applications. |
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Keywords: | cytotoxicity degradability hydrogels polymer oxidation self-healing |
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