氧氟沙星在聚L-色氨酸修饰电极上的电化学行为及测定

氧氟沙星(ofloxacin,OFL)又名氟嗪酸,是第3代喹诺酮类药物,通过作用于细胞DNA螺旋酶的A亚单位,抑制DNA的合成和复制导致细菌死亡,具有高效广谱抗菌作用,是治疗呼吸道、泌尿道、肠道、皮肤、关节及软组织等感染的有效药物,而大剂量的服用可发生结晶尿,若不调整剂量,会使肝、肾功能减退、血药浓度增高等,因此测定药物中OFL的含量有重要意义.

Electrochemical behaviors of ofloxacin at the poly (L-tryptophan) modified electrode

The poly(L-tryptophan) modified electrode was prepared,and the electrochemical behavior of ofloxacin at the poly(L-tryptophan) modified electrode was studied. Electrochemical experimental results showed that the electrochemical behavior of OFL in pH 5. 0 Britton-Robinson Buffer Solution( B-R) was an irreversible electrochemical oxidation process at PLT modified electrode and that the oxidation peak potential (E_(pa)) was 0. 950V. Within the scanning rate of 40～400 mV·s~(-1) ,the oxidation peak current had a linear relationship with scanning rate, indicating that the voltammetric behavior of OFL at the PLT modified electrode was an adsorption-limited electrode process. The variations of the oxidation peak currents with OFL concentration were examined by CV and it was found that a linear relationship existed between them in the range of 5. 53 × 10~(-6)～1.11 × 10~(-6)mol·L~(-1). The correlation coefficient was - 0. 9978, and the detection limit was 8. 65 ×10~(-8) mol·L~(-1). The recovery was in the range of 93.4%～107. 8% , The proposed method was applied to the determination of OFL in tablet samples.