How to select equivalent and complimentary reversed phase liquid chromatography columns from column characterization databases |
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Authors: | Endler M Borges |
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Institution: | Universidade Estadual de Campinas, Instituto de Química, Rua Monteiro Lobato, Cidade Universitária “Zeferino Vaz”, Distrito de Barão Geraldo, Campinas, SP, Caixa Postal 6154, CEP 13083-970, Brazil |
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Abstract: | Three RP-LC column characterization protocols Tanaka et al. (1989), Snyder et al. (PQRI, 2002), and NIST SRM 870 (2000)] were evaluated using both Euclidian distance and Principal Components Analysis to evaluate effectiveness at identifying equivalent columns. These databases utilize specific chromatographic properties such as hydrophobicity, hydrogen bonding, shape/steric selectivity, and ion exchange capacity of stationary phases. The chromatographic parameters of each test were shown to be uncorrelated. Despite this, the three protocols were equally successful in identifying similar and/or dissimilar stationary phases. |
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Keywords: | Column characterization Similar and dissimilar columns Revered phase liquid chromatography (RPLC) Principal Component Analysis (PCA) |
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