Integration of a highly ordered gold nanowires array with glucose oxidase for ultra-sensitive glucose detection |
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| Authors: | Jiewu Cui Samuel B Adeloju Yucheng Wu |
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| Institution: | 1. NanoScience and Sensor Technology Research Group, School of Applied Sciences and Engineering, Monash University, Gippsland Campus, Churchill 3842, VIC Australia;2. Laboratory of Functional Nanomaterials and Devices, School of Materials Science and Engineering, Hefei University of Technology, Hefei 230009, Anhui, China |
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| Abstract: | A highly sensitive amperometric nanobiosensor has been developed by integration of glucose oxidase (GOx) with a gold nanowires array (AuNWA) by cross-linking with a mixture of glutaraldehyde (GLA) and bovine serum albumin (BSA). An initial investigation of the morphology of the synthesized AuNWA by field emission scanning electron microscopy (FESEM) and field emission transmission electron microscopy (FETEM) revealed that the nanowires array was highly ordered with rough surface, and the electrochemical features of the AuNWA with/without modification were also investigated. The integrated AuNWA–BSA–GLA–GOx nanobiosensor with Nafion membrane gave a very high sensitivity of 298.2 μA cm−2 mM−1 for amperometric detection of glucose, while also achieving a low detection limit of 0.1 μM, and a wide linear range of 5–6000 μM. Furthermore, the nanobiosensor exhibited excellent anti-interference ability towards uric acid (UA) and ascorbic acid (AA) with the aid of Nafion membrane, and the results obtained for the analysis of human blood serum indicated that the device is capable of glucose detection in real samples. |
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| Keywords: | Gold nanowires array Glucose Glucose oxidase Nanobiosensor Amperometry Cross-linking immobilization |
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