Separation of phospholipids by capillary zone electrophoresis with indirect ultraviolet detection |
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| Authors: | Gao Fei Dong Juan Li Wei Wang Tao Liao Jie Liao Yiping Liu Huwei |
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| Affiliation: | Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Department of Chemistry, Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China. |
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| Abstract: | A simple method for separation of different anionic and zwitterionic phospholipid classes by capillary zone electrophoresis (CZE), using indirect UV detection with adenosine monophosphate (AMP) as background electrolyte and the UV-absorbing additive, was successfully developed in this study. The separation conditions including apparent pH (pH*) of running buffer, concentration of AMP, organic solvent, applied voltage and capillary temperature were systematically optimized. The application of this method to human blood sample was also briefly examined. |
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