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Possibilities and limitations of different modes of capillary electrophoresis for the quantitative determination of catechols and caffeine in black and green tea
Authors:L A Kartsova  O V Ganzha  A V Alekseeva
Institution:(1) Laboratory of Experimental Endocrinology, University of Crete, School of Medicine, P.O. Box 2208, 71003 Heraklion, Greece;(2) Laboratory of Gastroenterology, University of Crete, School of Medicine, 71003 Heraklion, Greece;
Abstract:Methods of capillary electrophoresis (capillary zone electrophoresis, micellar electrokinetic chromatography, and microemulsion electrokinetic chromatography) were compared in determining polyphenolic antioxidants and alkaloids in black and green tea. It was shown that reverse-flow micellar electrokinetic chromatography (RF-MEKC, pH < 2.5) using reversed pola rity is most preferable for the rapid simultaneous determination of catechols and caffeine. The detection limit was 1 mg/L.
Keywords:
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