| 维生素A诱导Shope肿瘤细胞凋亡 |
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| 引用本文： | 李咏梅,邵世和,陈曦,曾常茜,邱友红.维生素A诱导Shope肿瘤细胞凋亡[J].北华大学学报(自然科学版),1998(2). |
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| 作者姓名： | 李咏梅 邵世和 陈曦 曾常茜 邱友红 |
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| 作者单位： | 吉林医学院微生物教研室!吉林132001(李咏梅，邵世和，陈曦)，吉林医学院免疫教研室(曾常茜)，牡丹江农管局中心医院(邱友红) |
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| 摘 要： | 目的：探讨维生素A抑制Shope肿瘤细胞生长的机理。方法：电镜观察Shope肿瘤细胞凋亡的形态学改变：琼脂糖凝胶电泳进行DNA片段化；流式细胞仪检测凋亡细胞的百分比。结果：电镜下可VA诱导的Shope肿瘤细胞变小，细胞浆固缩，染色质聚集并靠近核膜，在1．5％琼脂糖凝胶电泳中，Shope肿瘤细胞DNA呈现明显的阶梯状图谱。流式细胞仪检测经10－5MV作用24h、48h、72h后，凋亡细胞百分比分别为：17．1％、22．4％、32．4％。结论：以上结果表明，VA对Shope肿瘤细胞有明显的抑制作用。
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| 关 键 词： | 维生素A Shope肿瘤细胞 细胞凋亡 |
Vitamin A induced Shope carcinoma cells apoptosis |
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| Li Yongmei, Shao ahihe, Chen xi, Zeng Chang qian.Vitamin A induced Shope carcinoma cells apoptosis[J].Journal of Beihua University(Natural Science),1998(2). |
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| Authors: | Li Yongmei Shao ahihe Chen xi Zeng Chang qian |
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| Abstract: | Objective:To study the mechanism of VA inhibted shope carinoma cells growth.Method:The mophologic changes in shope carinoma cells apoptosis were observed by eletronmicroscope.DNA fragments were prepared by agar egl eletronphorese and the percentages of apoptosis were detected by flow cytometer.Results:Under electronmicroscope:shope carcinoma cells induced by VA became small,Cytoplasm shrinked,chromosome assembled and closed to nuclear membrane.Obvious DNA ladder appeared in 1.5% agar gel electeronphores.After 24t.48h and 72h with 10-5 MVA the percentages of apoptosis were 17.1% 22.4% 32.4% respectively by flow cytometer.Conclnsion:The results showed tat VA had obvious inhibition to shope carcinoma cells. |
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| Keywords: | V_A shope carcinoma cells Apoptosis |
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