High-performance liquid chromatographic investigation of the enantioselectivity and mechanism of chiral recognition of new cholic acid-based stationary phases |
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Authors: | L. Vaton-Chanvrier Y. Combret J. C. Combret |
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Affiliation: | (1) IRCOF, Sciences et Méthodes Séparatives, UPRES EA 2659, Université de Rouen, 76821 Mont Saint Aignan Cedex, France |
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Abstract: | Summary To elucidate the mechanism of chiral recognition of cholic acid-based stationary phases, four new cholic acid derivatives, with differently substituted carbamate or three acetoxy groups, were bonded to a hydrosilyl-modified silica gel. Their capacity to discriminate between enantiomers was evaluated in normal-phase high-performance liquid chromatography. The results were compared with those from equivalent separations on trihydroxy- and 3α-phenylcarbamate-substituted cholic acid-based bonded phases. The influence of mobile phase composition of the separation of the enantiomers of amino alcohols was shown. Different mechanisms of chiral discrimination are discussed, highlighting the influence of the nature of the carbamate on enantioselectivity. |
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Keywords: | Column liquid chromatography New bonded stationary phases Mechanism of chiral separation Cholic acid derivatives |
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