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Analysis of the Lipidome of Xenografts Using MALDI-IMS and UHPLC-ESI-QTOF |
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| Authors: | Roberto Fernández Sergio Lage Beatriz Abad-García Gwendolyn Barceló-Coblijn Silvia Terés Daniel H López Francisca Guardiola-Serrano M Laura Martín Pablo V Escribá José A Fernández |
| Institution: | 1. Department of Physical Chemistry, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), Barrio Sarriena s/n, 48940, Leioa, Spain 2. Metabolism Unit, Department of Paediatrics, Cruces University Hospital, Plaza de Cruces s/n, 48903, Barakaldo, Vizcaya, Spain 3. Central Analysis Service, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), Barrio Sarriena s/n, 48940, Leioa, Spain 4. Lipids in Human Pathology, Research Unit, Hospital Universitari Son Espases, Palma Health Research Institute, Carretera de Valldemossa 79, E-07010, Palma, Balearic Islands, Spain 5. Unité de recherche Inserm 0916, Institut européen de chimie et biologie (IECB)-INSERM, 2, rue Robert Escarpit, 33607, Pessac, France 6. Laboratory of Molecular Cell Biomedicine, Department of Biology, University Institute for Research into Health Sciences (IUNICS), University of the Balearic Islands, 07122, Palma, Balearic Islands, Spain 7. Laboratory of Signal Transduction, Memorial Sloan-Kettering Cancer Center, 415 East 68th Street, New York, NY, 10065, USA |
| Abstract: | Human tumor xenografts in immunodeficient mice are a very popular model to study the development of cancer and to test new drug candidates. Among the parameters analyzed are the variations in the lipid composition, as they are good indicators of changes in the cellular metabolism. Here, we present a study on the distribution of lipids in xenografts of NCI-H1975 human lung cancer cells, using MALDI imaging mass spectrometry and UHPLC-ESI-QTOF. The identification of lipids directly from the tissue by MALDI was aided by the comparison with identification using ESI ionization in lipid extracts from the same xenografts. Lipids belonging to PCs, PIs, SMs, DAG, TAG, PS, PA, and PG classes were identified and their distribution over the xenograft was determined. Three areas were identified in the xenograft, corresponding to cells in different metabolic stages and to a layer of adipose tissue that covers the xenograft. ? |
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